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Biotechnology, XII: Model Paper

Mrs. Anu Vijayan, PGT(BioTech)

Model Paper –23

Class – XII

Subject – Biotechnology 

Time allowed : 3 hours              Maximum Marks: 70 

General Instructions :

          (i) All questions are compulsory.

          (ii) There is no overall choice. However, an internal choice has been provided in one

          question of two marks and two questions of five marks. You have to attempt only

          one of the choices in such questions. Question paper contains four sections -A, B, C

          and D.

          (iii) Question numbers 1 to 5 are very short answer questions, carrying 1 mark each.

          (iv) Question numbers 6 to 15 are short answer questions, carrying 2 marks each.

          (v) Question numbers 16 to 25 are also short answer questions, carrying 3 marks each.

          (vi) Question numbers 26 to 28 are long answer questions, carrying 5 marks each.

          (vii) Use of calculators is not permitted. However, you may use log tables, if necessary.

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                  SECTION A (1 marks each) 

  1. Why sickle celled anemia is called a molecular disease?
  2. Give one characteristics feature of type-II restriction enzyme.
  3. Name any two molecular markers.
  4. What kind of data can be accessed from the data retrieval tool called Taxonomic browser?
  5. Give the term which can be used for the organ grafts transferred between a donor & a recipient.


                  SECTION B ( 2 marks each) 

  1.   Give the advantage and disadvantage of serum containing media.
  2. What are the factors governing the stability of karyotype?
  3. From a given group of crop plants how will you diagnose the diseased plants?  Explain any one technique.


  1. From the given table determine which organism has maximum doubling time and why?                         

                     Microorganisms                                           specific growth rate (h)

                     Aspergillus nidulans                                            0.36

                     Penicillium chrysogenum                                    0.12

                     Benechean atriegans                                             4.24

                     Methylomonas methenolytica                           0.12 

  1. Name the device commonly used for continuous culture technique. Name the two   factors that control the working of a chemostat.


  1. Expand the term BLAST .Give the common use of the following programmers (i) BLASTp (ii)TBLASTn.


  1. Which vectors are used in human genome sequencing & why?


  1. Explain the blue white method of screening the recombinant plasmid?


  1. A single cell of E.coli  is supposed to produce about 2000 different proteins.


  1. under optimum conditions. One of the desired intercellular enzymes produced is in the form of 2500 molecules/cells. If the molecular weight of that enzyme is 100000D.How many E.coli cells are required to produce 1gm of enzymes.


  1. What are secondary metabolites? Name the secondary metabolites produced by the following plants. (a)Atropa beladona (b) Cinchona officinalis.


   SECTION C (3 marks each) 

16.What is the principle behind the working of a mass spectrometer? Explain the various components of it. 

17.Define essential amino acids. What is the importance of BCAA in athletes. Which protein has highest amount of BCAA. 

18. With the help of schematic explain the method for constructing a cDNA library .What are the advantage of cDNA over genomic DNA? 

19. Give at least five uses of bioinformatics tools in analysis of biological data. 

20. Who pioneered the dideoxy chain termination method .What are its requirements .Explain the procedure in detail. 

21. Explain the two methods of strain improvement of microorganism with one example of each. 

22.Explain the various steps of plant regeneration .What factors affect the regeneration process? 

23. What is ELISA? Write the steps of ELISA with a neat labeled diagram.   

24. How can male sterile plants are produced by genetic Manipulation .What is the importance

        of producing such plants? Name the enzyme which restores male fertility. 

25. What are the expressed sequence tags? How can the EST approach be used in genome 

        sequencing projects? What are the advantages of this approach? 

      SECTION D (5 marks each) 

26. (i) What are stem cells?

         (ii) What are the various types of stem cells?

         (iii) Explain the repopulation assay to identify the haematopoetic stem cells. 

27. Give the detailed account of recombinant DNA technology. 

        Why yeasts are commonly used as host for expression of eukaryotic genes. 


27.   What are cloning vectors?

         Give the detailed account of different bacterial plasmids as cloning vectors. 

28.   What do you understand by proteomics? List the various types of proteomics?


         Give an account of therapeutic proteins, neutraceutical proteins and non catalytic protein.


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